brxA [2017-8-11 16:0:3]

bacilliredoxin

locus: BSU21860

pI: 4.62

mw: 15.73 kDa

protein length: 144 aa

gene length: 432 bp

function: de-bacillithiolation of S-bacillithiolated OhrR and MetE

product: bacilliredoxin

essential: no

synonyms: yphP

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,300,221 → 2,300,655

The protein

Catalyzed reaction/ biological activity

• de-bacillithiolation of S-bacillithiolated [protein|7A3A8AF7728474204396BB7A548F747D28E6FAC7|OhrR] and [protein|23F405D5B849E4EDC1D599F86C71DFD2E55C0305|MetE] [Pubmed|24313874]

Protein family

• scpA family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|8B134BDC82C92DB7747A3AF291C96BB0F223D0CF|BrxB]

[SW|Domains]

• contains an CxC redox motif [Pubmed|20308541]

Modification

• S-bacillithiolation upon hypochlorite stress on Cys-53 [Pubmed|21749987]

Structure

• [PDB|3FHK] [Pubmed|19653655]

Expression and Regulation

Operons

• genes: [gene|FBE3C55526FE0CBBDA71AE0B0D7C6AD074E8A63E|brxA]
  description: [Pubmed|22383849]

• genes: [gene|FBE3C55526FE0CBBDA71AE0B0D7C6AD074E8A63E|brxA]-[gene|4E06C3D56DC798E19DD166CEE4921EB8F1DC0168|ypiP]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A879 (yphP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/879 NBRP B. subtilis, Japan]
• CS213 (''[gene|FBE3C55526FE0CBBDA71AE0B0D7C6AD074E8A63E|brxA]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]

References

• 22938038,19653655,20308541,21749987,24313874